how about the toxicity from the ARMMs? e.g. delivery of WT p53 into mice may cause apoptosis in cells in normal tissues.

What would you predict to happen when you have mixtures of metals - arsenic + something else (e.g., Mn or Chromium)?

Would you expect same pattern as arsenic?

Great -thanks

Are other non-coding RNAs being investigated by your group as part of the research in EVs?

have you examined what sort of EVs are favored for release following metal exposures (exosomes, ARMMs, microvesicles)?

How do exosomes contribute or not to your studies

do ARMM EVs differ from exosomes mainly based on the presence of the ARRCD protein or are there other different cargo that stand out between these two types of EVs?

How are ARMMs primarily incorporated by recipient cells? Is there an activation of clathrin/calveolin-mediated endocytosis or are then incorporated by plasma membrane fusion?

Have you used any specific antibody-based column purification strategies to differentiate exosomes from ARMMs

How is the functional association or connection between ARRCD 1 and ARRCD1 may like? Since they seem like to play kind of specific roles in regulating the process of virus budding ?

Once ARMMs are internalized, are they targeted to lysosome or endosome of the recipient cells

Sorry ARRCD3

I saw that overexpression of ARRDC1 increases EV release by cells. Is there an increase in ARRDC1 in cells following metal exposure?

Outstanding work and look forward to learning more about the ARMMs, exosomes in metals toxicology in the future. Thank you

Thank you!